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Characterization of an ethylene-induced esterase gene isolated from Citrus sinensis by competitive hybridization

Identifieur interne : 002E60 ( Main/Exploration ); précédent : 002E59; suivant : 002E61

Characterization of an ethylene-induced esterase gene isolated from Citrus sinensis by competitive hybridization

Auteurs : GUANG YAN ZHONG [Israël] ; Raphael Goren [Israël] ; Joseph Riov [Israël] ; Edward C. Sisler [États-Unis] ; Doron Holland [Israël]

Source :

Physiologia Plantarum : (København. 1948) [ 0031-9317 ] ; 2001.

RBID : Pascal:01-0458560

Descripteurs français

Pascal (Inist)
- Traitement substance croissance, Expression génique, Gène, Esterases, Séquence aminoacide, Homologie, Caractérisation, Méthode immunologique, Citrus sinensis, Ethylène, Activité enzymatique.

English descriptors

KwdEn :
- Aminoacid sequence, Characterization, Citrus sinensis, Enzymatic activity, Esterases, Ethylene, Gene, Gene expression, Growth regulator treatment, Homology, Immunological method.

Abstract

A simple new method, competitive hybridization, for identification of differentially regulated genes was used to isolate novel genes induced by ethylene in citrus (Citrus sinensis [L.] Osbeck cv. Shamouti) leaves. One of the isolated genes, an ethylene-induced esterase gene (EIE), was further characterized. The deduced protein sequence of this gene shows a similarity to those of several plant α/β hydrolase gene family members, which are known to be involved in secondary metabolism. Northern blot analysis demonstrated that EIE mRNA was induced by ethylene within 4 h and accumulated to a very high level 24 h after the initiation of ethylene treatment. Induction of EIE by ethylene could be counteracted by 1-methylcyclopropene, a potent ethylene perception inhibitor, indicating that the expression of EIE is ethylene-dependent. The bacterially expressed protein of EIE was recognized by antiserum against Pir7b, a naphthol AS esterase induced in rice by the non-host pathogen, Pseudomonas syringae pv. syringae. The EIE protein was identified in ethylene-treated leaves using anti-Pir7b antibodies. An α-naphthyl acetate esterase accumulated concomitantly with the increase in EIE protein in ethylene-treated citrus leaves. An enzyme activity assay followed by western analysis confirmed that the esterase was EIE.

Affiliations:

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Le document en format XML

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<front><div type="abstract" xml:lang="en">A simple new method, competitive hybridization, for identification of differentially regulated genes was used to isolate novel genes induced by ethylene in citrus (Citrus sinensis [L.] Osbeck cv. Shamouti) leaves. One of the isolated genes, an ethylene-induced esterase gene (EIE), was further characterized. The deduced protein sequence of this gene shows a similarity to those of several plant α/β hydrolase gene family members, which are known to be involved in secondary metabolism. Northern blot analysis demonstrated that EIE mRNA was induced by ethylene within 4 h and accumulated to a very high level 24 h after the initiation of ethylene treatment. Induction of EIE by ethylene could be counteracted by 1-methylcyclopropene, a potent ethylene perception inhibitor, indicating that the expression of EIE is ethylene-dependent. The bacterially expressed protein of EIE was recognized by antiserum against Pir7b, a naphthol AS esterase induced in rice by the non-host pathogen, Pseudomonas syringae pv. syringae. The EIE protein was identified in ethylene-treated leaves using anti-Pir7b antibodies. An α-naphthyl acetate esterase accumulated concomitantly with the increase in EIE protein in ethylene-treated citrus leaves. An enzyme activity assay followed by western analysis confirmed that the esterase was EIE.</div>
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Characterization of an ethylene-induced esterase gene isolated from Citrus sinensis by competitive hybridization

Characterization of an ethylene-induced esterase gene isolated from Citrus sinensis by competitive hybridization

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri